Contact an Account ManagerHPLC quantitation of cyclooxygenase and lipoxygenase metabolites of arachidonic acid from rat polymorphonuclear leukocytes
Summary
Type: HPLC
Application #: 100360
Catalog#: 720949.46 - MN HPLC Column, Nucleosil C18, 100A, 3um, 4.6x150mm
Detection: UV
Conditions: eluent A: 0.05M NaH2PO4 pH 5 - acetonitrile (72.5:27.5, v/v),
eluent B: 0.05M NaH2PO4 pH 5 -methanol - acetonitrile (30:35:35, v/v),
100% A isocratic for 20min (205nm), then linear gradient from 100% A to 100% B in 25min (280nm), finally 100% B for 20min (230nm),
flow rate: 1ml/min
Authors: Tordjman C
Source: J Chromatogr 532 (1990) 135 - 143
Details
Legend: Elution pattern in a single run of cyclooxygenase and lipoxygenase products
(synthetic standards).